ad****@ws****.edu & No phone number available.

Adam Johnson

Ph.D. in Molecular Biosciences, with an emphasis on protein engineering, enzymatic activity analysis and therapeutic evaluation Molecular and cellular... | Seattle, Washington, United States

*50 free lookup(s) per month.

No credit card required.

Adam Johnson’s Emails ad****@ws****.edu

Adam Johnson’s Phone Numbers No phone number available.

Social Media

Adam Johnson’s Location Seattle, Washington, United States

Adam Johnson’s Expertise Ph.D. in Molecular Biosciences, with an emphasis on protein engineering, enzymatic activity analysis and therapeutic evaluation Molecular and cellular assay specialist with experience in evaluating, designing and implementing work plans geared toward the analysis of novel protein and cellular based therapeutics TECHNICAL EXPERTISE Molecular Biology: Protein engineering strategies (site-directed, error-prone and regio-specific mutagenesis); Library construction/screening; Experience designing and implementing bacterial selection systems for mutagenic libraries; Molecular cloning; PCR techniques; Reverse-Transcriptase PCR; Primer design; Gel electrophoresis; Microbiological techniques; Lentiviral vector design and production; promoter design Cell biology: Flow cytometry: multicolor flow cytometry, fluorescence-based cell sorting, use of DiVA, FlowJo software; Cytokine production assays (luminex, ELISA); Mammalian cell culture (adherent, suspension, primary T cell); conditional promoter luciferase reporter assay; Mammalian cell transfection and transduction; phospho-protein specific immunoblot; in vitro cytotoxicity assay; antigen-presenting cell assay (chromium release assay); in vivo tumor model for cancer drug evaluation (xenograft, subcutaneous, intraperitoneal, intravenous, intrathecal); Proliferation assays (CFSE); Cell harvesting; TFF (tangential flow filtration); Cell fractionation and isolation; Miltenyi magnetic cell separation (AutoMacs) Biochemistry: Protein purification, quantification, and characterization; Column purification (Anion exchange, SEC) using an AKTApurifier; Ni-NTA affinity; Protein A; SDS-PAGE; Enzymatic assays (radioactive and spec based); Bradford assay; BCA assay; Octet (SPR); Protein structure visualization (Chimera)

Adam Johnson’s Current Industry Sana Biotechnology

Not the Adam Johnson you were looking for?

Find accurate emails & phone numbers for over 700M professionals.

Work Experience

Sana Biotechnology

Senior Director, T Cell Immunology

Mon May 01 2023 00:00:00 GMT+0000 (Coordinated Universal Time) — Present

Sana Biotechnology

Principal Scientist (Group Lead)

Mon Feb 01 2021 00:00:00 GMT+0000 (Coordinated Universal Time) — Mon May 01 2023 00:00:00 GMT+0000 (Coordinated Universal Time)

Seattle Childrens Research Institute

Manager R&D Immunology

Fri Jan 01 2016 00:00:00 GMT+0000 (Coordinated Universal Time) — Fri Jan 01 2021 00:00:00 GMT+0000 (Coordinated Universal Time)

Seattle Childrens Research Institute

Research Scientist IV

Wed Jan 01 2014 00:00:00 GMT+0000 (Coordinated Universal Time) — Fri Jan 01 2016 00:00:00 GMT+0000 (Coordinated Universal Time)

Seattle Childrens Research Institute

Fellow - PhD

Sun Jan 01 2012 00:00:00 GMT+0000 (Coordinated Universal Time) — Wed Jan 01 2014 00:00:00 GMT+0000 (Coordinated Universal Time)

Seattle Genetics

Graduate Student Intern

Thu Jul 01 2010 00:00:00 GMT+0000 (Coordinated Universal Time) — Fri Oct 01 2010 00:00:00 GMT+0000 (Coordinated Universal Time)

Washington State University

Graduate Student

Tue Aug 01 2006 00:00:00 GMT+0000 (Coordinated Universal Time) — Tue Nov 01 2011 00:00:00 GMT+0000 (Coordinated Universal Time)

Targeted Genetics

Process Development

Tue Mar 01 2005 00:00:00 GMT+0000 (Coordinated Universal Time) — Sat Jul 01 2006 00:00:00 GMT+0000 (Coordinated Universal Time)

Ttm Technologies

Quality Control

Thu Jan 01 2004 00:00:00 GMT+0000 (Coordinated Universal Time) — Sat Jan 01 2005 00:00:00 GMT+0000 (Coordinated Universal Time)

Skills

Languages

Adam Johnson Email Addresses

ad****@ws****.edu Work Email

ad****@ws****.edu Personal Email

ad****@sa****.com Personal Email

FAQ About Adam Johnson

Adam Johnson is located at
Seattle, Washington, United States

Adam Johnson is a
Ph.D. in Molecular Biosciences, with an emphasis on protein engineering, enzymatic activity analysis and therapeutic evaluation Molecular and cellular assay specialist with experience in evaluating, designing and implementing work plans geared toward the analysis of novel protein and cellular based therapeutics TECHNICAL EXPERTISE Molecular Biology: Protein engineering strategies (site-directed, error-prone and regio-specific mutagenesis); Library construction/screening; Experience designing and implementing bacterial selection systems for mutagenic libraries; Molecular cloning; PCR techniques; Reverse-Transcriptase PCR; Primer design; Gel electrophoresis; Microbiological techniques; Lentiviral vector design and production; promoter design Cell biology: Flow cytometry: multicolor flow cytometry, fluorescence-based cell sorting, use of DiVA, FlowJo software; Cytokine production assays (luminex, ELISA); Mammalian cell culture (adherent, suspension, primary T cell); conditional promoter luciferase reporter assay; Mammalian cell transfection and transduction; phospho-protein specific immunoblot; in vitro cytotoxicity assay; antigen-presenting cell assay (chromium release assay); in vivo tumor model for cancer drug evaluation (xenograft, subcutaneous, intraperitoneal, intravenous, intrathecal); Proliferation assays (CFSE); Cell harvesting; TFF (tangential flow filtration); Cell fractionation and isolation; Miltenyi magnetic cell separation (AutoMacs) Biochemistry: Protein purification, quantification, and characterization; Column purification (Anion exchange, SEC) using an AKTApurifier; Ni-NTA affinity; Protein A; SDS-PAGE; Enzymatic assays (radioactive and spec based); Bradford assay; BCA assay; Octet (SPR); Protein structure visualization (Chimera) Sana Biotechnology

Adam Johnson currently works in the
Sana Biotechnology

Adam Johnson specializes in
Ph.D. in Molecular Biosciences, with an emphasis on protein engineering, enzymatic activity analysis and therapeutic evaluation Molecular and cellular assay specialist with experience in evaluating, designing and implementing work plans geared toward the analysis of novel protein and cellular based therapeutics TECHNICAL EXPERTISE Molecular Biology: Protein engineering strategies (site-directed, error-prone and regio-specific mutagenesis); Library construction/screening; Experience designing and implementing bacterial selection systems for mutagenic libraries; Molecular cloning; PCR techniques; Reverse-Transcriptase PCR; Primer design; Gel electrophoresis; Microbiological techniques; Lentiviral vector design and production; promoter design Cell biology: Flow cytometry: multicolor flow cytometry, fluorescence-based cell sorting, use of DiVA, FlowJo software; Cytokine production assays (luminex, ELISA); Mammalian cell culture (adherent, suspension, primary T cell); conditional promoter luciferase reporter assay; Mammalian cell transfection and transduction; phospho-protein specific immunoblot; in vitro cytotoxicity assay; antigen-presenting cell assay (chromium release assay); in vivo tumor model for cancer drug evaluation (xenograft, subcutaneous, intraperitoneal, intravenous, intrathecal); Proliferation assays (CFSE); Cell harvesting; TFF (tangential flow filtration); Cell fractionation and isolation; Miltenyi magnetic cell separation (AutoMacs) Biochemistry: Protein purification, quantification, and characterization; Column purification (Anion exchange, SEC) using an AKTApurifier; Ni-NTA affinity; Protein A; SDS-PAGE; Enzymatic assays (radioactive and spec based); Bradford assay; BCA assay; Octet (SPR); Protein structure visualization (Chimera)

Adam Johnson speaks the following languages:
No languages available.

Adam Johnson has prior experience in the following industries:
Sana Biotechnology , Seattle Childrens Research Institute , Seattle Childrens Research Institute , Seattle Childrens Research Institute , Seattle Genetics , Washington State University , Targeted Genetics , Ttm Technologies

Adam Johnson has the following professional experience:
Sana Biotechnology , Sana Biotechnology , Seattle Childrens Research Institute , Seattle Childrens Research Institute , Seattle Childrens Research Institute , Seattle Genetics , Washington State University , Targeted Genetics , Ttm Technologies .