
Varsha Chauhan
• Experience with small-molecule drugs, Cell-based therapy using miRNA, Immunotherapy (cytokine~toxin fusion). • Hands on with molecular, mammalian cell culture and... | San Bruno, California, United States
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Varsha Chauhan’s Emails va****@wi****.com
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Varsha Chauhan’s Location San Bruno, California, United States
Varsha Chauhan’s Expertise • Experience with small-molecule drugs, Cell-based therapy using miRNA, Immunotherapy (cytokine~toxin fusion). • Hands on with molecular, mammalian cell culture and microbiological experiments according to protocols/SOPs in the aseptic laboratory conditions in Biosafety Level-1/2. • Conduct mammalian cell culture and tissue culture experiments in GMP facility, including cell culture growth, cell passaging, cell counting, and cell harvesting. • Perform standard molecular experiments, including Plasmid DNA purification (mini-prep and maxi-prep), PCR, Gene cloning using Gibson/Gateway methods, Clone screening, sample preparation for DNA sequencing, designing oligos using Gene Runner, Analyzing DNA sequence. • Experience with bacterial expression using shake flask for small-scale recombinant protein expression. • Establishment of protein purification workflow of recombinant proteins using AKTA followed by protein analysis using SDS-PAGE and western blotting. • Skillful in biochemical, biophysical and structural studies of target proteins using Mass Spectrometry, X-ray Crystallography, UV/Vis-Spectroscopy and ELISA. • High throughput Cell culture-based assays: cytotoxicity study and Vascular Leak toxicity using fluorescence. • Data analysis using Prism software, data entry in ELN and presentation of data in laboratory meetings. • Effective interpersonal skills, including the ability to think scientifically, work independently and collaboratively in a fast-paced, multidisciplinary laboratory environment.
Varsha Chauhan’s Current Industry Wildtype
Varsha
Chauhan’s Prior Industry
National Institute Of Criminology And Forensic Science
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Division Of Forensic Medicine Aiims
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Lacones Ccmb
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Department Of Anthropology University Of Delhi North Campus
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University Of Maryland Baltimore County
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Johns Hopkins University
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Jamia Hamdard
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Wildtype
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Work Experience

Wildtype
Research Associate, Cell Culture Manufacturing
Mon Aug 01 2022 00:00:00 GMT+0000 (Coordinated Universal Time) — Tue Nov 01 2022 00:00:00 GMT+0000 (Coordinated Universal Time)
Jamia Hamdard
Researcher
Thu Nov 01 2018 00:00:00 GMT+0000 (Coordinated Universal Time) — Thu Jul 01 2021 00:00:00 GMT+0000 (Coordinated Universal Time)
Johns Hopkins University
Research Technologist
Tue Sep 01 2015 00:00:00 GMT+0000 (Coordinated Universal Time) — Thu Mar 01 2018 00:00:00 GMT+0000 (Coordinated Universal Time)
University Of Maryland Baltimore County
Visiting Scientist
Sun Jan 01 2012 00:00:00 GMT+0000 (Coordinated Universal Time) — Sat Dec 01 2012 00:00:00 GMT+0000 (Coordinated Universal Time)
Department Of Anthropology University Of Delhi North Campus
Project Assistant
Wed Jul 01 2009 00:00:00 GMT+0000 (Coordinated Universal Time) — Tue Feb 01 2011 00:00:00 GMT+0000 (Coordinated Universal Time)
Lacones Ccmb
Short-Term Traine
Sun Feb 01 2009 00:00:00 GMT+0000 (Coordinated Universal Time) — Sun Mar 01 2009 00:00:00 GMT+0000 (Coordinated Universal Time)
Division Of Forensic Medicine Aiims
Short-Term Traine
Thu May 01 2008 00:00:00 GMT+0000 (Coordinated Universal Time) — Sun Jun 01 2008 00:00:00 GMT+0000 (Coordinated Universal Time)
National Institute Of Criminology And Forensic Science
Student In Dissertation Project
Fri Jun 01 2007 00:00:00 GMT+0000 (Coordinated Universal Time) — Wed Jul 01 2009 00:00:00 GMT+0000 (Coordinated Universal Time)